CAS Registry Number: 75-09-2
Substance Name: Methylene chloride
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Molecular Formula: CH2Cl2 Beilstein Registry Number: 1730800 Synonyms: Aerothene MM; Chlorure de methylene (French); Dichloromethane (DOT:OSHA); Methane dichloride; Methylene bichloride; Methylene chloride (ACGIH:DOT:OSHA); Methylene dichloride; Metylenu chlorek (Polish); Narkotil; NCI-C50102; R 30; R30 (refrigerant); RCRA waste number U080; Solaesthin; Solmethine; UN1593 (DOT) Compound Class: Agricultural Chemical; Tumorigen; Drug; Mutagen; Reproductive
Effector; Human Data; Primary Irritant IRRITATION DATA: Skin Rabbit 810 mg/24H Severe EJTXAZ 9,171,1976 Skin Rabbit 100 mg/24H Moderate 85JCAE -,88,1986 Eye Rabbit 162 mg Moderate EJTXAZ 9,171,1976 Eye Rabbit 10 mg Mild TXCYAC 6,173,1976 Eye Rabbit 500 mg/24H Mild 85JCAE -,88,1986 MUTATION DATA: Mutation in microorganisms Salmonella typhimurium 5700 ppm MUREAV 56,245,1978 Mutation in microorganisms Escherichia coli 6300 ppm EMMUEG 20,211,1992 Phage inhibition capacity Escherichia coli 8250 ug/well MUREAV 260,349,1991 Sex chromosome loss and nondisjunction Drosophila melanogaster Oral 125 mmol/L MUREAV 90,91,1981 DNA inhibition Human Fibroblast 5000 ppm/1H-C MUREAV 81,203,1981 Morphological transformation Rat Embryo 160 umol/L ITCSAF 14,290,1978 DNA damage Rat Oral 1275 mg/kg TCMUD8 9,61,1989 DNA damage Rat Liver 30 umol/L CRNGDP 15,991,1994 Micronucleus test Mouse Inhalation 27760 mg/m3/6H/2W-I EMMUEG 15,221,1990 DNA damage Mouse Liver 400 umol/L CRNGDP 15,991,1994 DNA damage Mouse Inhalation 4000 ppm/6H-C CRNGDP 15,991,1994 Cytogenetic analysis Mouse Inhalation 27760 mg/m3/6H/2W-I EMMUEG 15,221,1990 Sister chromatid exchange Mouse Inhalation 13880 mg/m3/6H/2W-I EMMUEG 15,221,1990 Morphological transformation Hamster Embryo 1300 uL/plate EVSRBT 25,75,1982 DNA damage Hamster Ovary 3000 ppm MUREAV 367,143,1996 DNA inhibition Hamster Lung 5000 ppm/1H-C MUREAV 81,203,1981 Other mutation test systems Hamster Ovary 6628 mg/L MUREAV 116,361,1983 Cytogenetic analysis Hamster Lung 1 umol/L TECSDY 13,205,1987 Cytogenetic analysis Hamster Ovary 6628 mg/L MUREAV 116,361,1983 Sister chromatid exchange Hamster Lung 5000 ppm/1H-C MUREAV 81,203,1981 Mutation in mammalian somatic cells Hamster Ovary 3000 ppm MUREAV 367,143,1996 REPRODUCTIVE EFFECTS DATA: Behavioral Inhalation Rat TCLo 4500 ppm/24H 1-17D preg TXAPA9 52,29,1980 Developmental Abnormalities: Musculoskeletal system;Developmental Abnormalities: Urogenital system Inhalation Rat TCLo 1250 ppm/7H 6-15D preg TXAPA9 32,84,1975 Developmental Abnormalities: Musculoskeletal system Inhalation Mouse TCLo 1250 ppm/7H 6-15D preg TXAPA9 32,84,1975 TUMORIGENIC EFFECTS DATA: Carcinogenic criteria; Endocrine--Tumors; Inhalation Rat TCLo 3500 ppm/6H/2Y-I FAATDF 4,30,1984 Carcinogenic criteria; Lungs, Thorax, or Respiration--Tumors; Inhalation Mouse TCLo 2000 ppm/5H/2Y-C NTPTR* NTP-TR-306,1986 TOXICITY EFFECTS DATA: Paresthesia;Somnolence (general depressed activity);Convulsions or effect on seizure threshold Oral Human LDLo 357 mg/kg 34ZIAG -,390,1969 Altered sleep time;Somnolence (general depressed activity);Change in rate Inhalation Human TCLo 500 ppm/1Y-I ABHYAE 43,1123,1968 Euphoria Inhalation Human TCLo 500 ppm/8H SCIEAS 176,295,1972 Ataxia Oral Rat LD50 1600 mg/kg FAONAU 48A,94,1970
Structural or functional change in trachea or bronchi;Acute pulmonary edema Intratracheal Rat LDLo 350 mg/kg NTIS** OTS0520615
Ulceration or bleeding from stomach;Ulceration or bleeding from small intestine Unreported Mouse LD50 4770 mg/kg ESKGA2 28,P31,1982
Miosis (pupillary constriction);BP lowering not characterized in autonomic section;Dyspnea Inhalation Dog LCLo 14108 ppm/7H NIHBAZ 191,1,1949 Liver function tests impaired Intraperitoneal Dog LDLo 950 mg/kg TXAPA9 10,119,1967
General anesthetic;Ataxia;Body temperature decrease Inhalation Cat LCLo 43400 mg/m3/4.5H AHBAAM 116,131,1936
OTHER MULTIPLE DOSE EFFECTS DATA: Fluid intake; Fatty liver degeneration; Weight loss or decreased weight gain; Oral Rat TDLo 91 gm/kg/2Y-C FCTOD7 24,951,1986 Lungs, Thorax, or Respiration--Other changes; Phosphatases; Dehydrogenases; Inhalation Rat TCLo 3700 ppm/5H/4W-I TOLED5 7,41,1980 Liver--Other changes; Changes in tubules; Changes in bladder weight; Inhalation Rat TCLo 5000 ppm/24H/14W-C NTIS** AD746-295 Changes in liver weight; Death in the "U" date type field; Inhalation Rat TCLo 8400 ppm/6H/13W-I NTPTR* NTP-TR-306,1986 Weight loss or decreased weight gain; Inhalation Rat TCLo 44 mg/m3/24H/96D-C GISAAA 40(9),105,1975 Liver--Other changes; Changes in liver weight; Skin Rat TDLo 39270 mg/kg/17W-I GTPZAB 26(5),41,1982 Changes in tubules; Oral Mouse TDLo 1862 mg/kg/2W-I DCTODJ 6,563,1983 Death in the "U" date type field; Inhalation Mouse TCLo 13000 ppm/6H/19D-I NTPTR* NTP-TR-306,1986 Changes in liver weight; Changes in Spleen weight; Other esterases; Inhalation Mouse TCLo 150 ppm/24H/30D-C APTOA6 59,73,1986 Changes in liver weight; Weight loss or decreased weight gain; Inhalation Mouse TCLo 5000 ppm/24H/7D-C TXAPA9 23,660,1972 Changes in liver weight; Changes in Spleen weight; Other esterases; Inhalation Mouse TCLo 300 ppm/24H/90D-C APTOA6 59,73,1986 Food intake (animal); Musculoskeletal--Other changes; Death in the "U" date type field; Inhalation Dog TCLo 1000 ppm/24H/7W-C NTIS** AD746-295 Food intake (animal); Weight loss or decreased weight gain; Death in the "U" date type field; Inhalation Monkey TCLo 5000 ppm/24H/32D-C NTIS** AD746-295 Liver--Other changes; Lipids including transport; Inhalation Guinea pig TCLo 552 ppm/6H/5D-I AIHAAP 37,345,1976 REVIEWS: ACGIH TLV-Animal carcinogen DTLVS* TLV/BEI,1997 ACGIH TLV-TWA 174 mg/m3 (50 ppm) DTLVS* TLV/BEI,1997 IARC Cancer Review:Animal Sufficient Evidence IMEMDT 41,43,1986 IARC Cancer Review:Human Inadequate Evidence IMEMDT 41,43,1986 IARC Cancer Review:Group 2B IMSUDL 7,194,1987 TOXICOLOGY REVIEW FAZMAE 18,365,1974 STANDARDS AND REGULATIONS: DOT-HAZARD 6.1; LABEL:KEEP AWAY FROM FOOD CFRGBR 49,172.101,1992 EPA FIFRA 1988 PESTICIDE SUBJECT TO REGISTRATION OR RE REGISTRATION FEREAC 54,7740,1989 MSHA STANDARD-air TWA 500 ppm (1750 mg/m3) DTLVS* 3,171,1971 OSHA PEL (Gen Indu) 8H TWA 500 ppm;CL 1000 ppm;Pk 2000 ppm/5M/2H CFRGBR 29,1910.1000,1994 OSHA PEL (Construc) see 56 FR 57036 CFRGBR 29,1926.55,1994 OSHA PEL (Shipyard) 8H TWA 500 ppm (1740 mg/m3 CFRGBR 29,1915.1000,1993 OSHA PEL (Fed Cont) 8H TWA 500 ppm (1740 mg/m3) CFRGBR 41,50-204.50,1994 OEL-AUSTRALIA TWA 100 ppm (350 mg/m3);Carcinogen JAN 1993 OEL-AUSTRIA TWA 100 ppm (360 mg/m3) JAN 1993 OEL-BELGIUM TWA 50 ppm (174 mg/m3);Carcinogen JAN 1993 OEL-DENMARK TWA 50 ppm (175 mg/m3);Skin;Carcinogen JAN 1993 OEL-FINLAND TWA 100 ppm (350 mg/m3);STEL 250 ppm (870 mg/m3) JAN 1993 OEL-FRANCE TWA 100 ppm (360 mg/m3);STEL 500 ppm (1800 mg/m3) JAN 1993 OEL-GERMANY TWA 100 ppm (360 mg/m3);Carcinogen JAN 1993 OEL-HUNGARY STEL 10 mg/m3;Carcinogen JAN 1993 OEL-JAPAN TWA 100 ppm (350 mg/m3) JAN 1993 OEL-THE NETHERLANDS TWA 100 ppm (350 mg/m3);STEL 500 ppm JAN 1993 OEL-THE PHILIPINES TWA 500 ppm (1740 mg/m3) JAN 1993 OEL-POLAND TWA 50 mg/m3 JAN 1993 OEL-RUSSIA TWA 100 ppm;STEL 50 mg/m3 JAN 1993 OEL-SWEDEN TWA 35 ppm (120 mg/m3);STEL 70 ppm (250 mg/m3);Skin JAN 1993 OEL-SWITZERLAND TWA 100 ppm (360 mg/m3);STEL 500 ppm JAN 1993 OEL-THAILAND TWA 500 mg/m3;STEL 1000 mg/m3 JAN 1993 OEL-TURKEY TWA 500 ppm (1740 mg/m3) JAN 1993 OEL-UNITED KINGDOM TWA 100 ppm (350 mg/m3);STEL 250 ppm JAN 1993 OEL IN BULGARIA COLOMBIA, JORDAN, KOREA check ACGIH TLV OEL IN NEW ZEALAND SINGAPORE, VIETNAM check ACGIH TLV NIOSH CRITERIA DOCUMENTS: NIOSH REL TO METHYLENE CHLORIDE air:CA lowest feasible concentration NIOSH* DHHS #92-100,1992 NOHS 1974: HZD 47270; NIS 374; TNF 89025; NOS 192; TNE 975696 NOES 1983: HZD 47270; NIS 363; TNF 87086; NOS 212; TNE 1438196; TFE 35 2536 NTP, NIOSH, EPA STATUS: ATSDR TOXICOLOGY PROFILE (NTIS** PB/89/194468/AS) EPA GENETOX PROGRAM 1988, Positive: Cell transform.-RLV F344 rat embryo EPA GENETOX PROGRAM 1988, Positive: Histidine reversion-Ames test EPA GENETOX PROGRAM 1988, Positive: S cerevisiae gene conversion; Scerevisiae-homozygosis EPA GENETOX PROGRAM 1988, Positive: S cerevisiae-reversion EPA GENETOX PROGRAM 1988, Negative: D melanogaster Sex-linked lethal EPA TSCA Section 8(b) CHEMICAL INVENTORY EPA TSCA 8(a) PRELIMINARY ASSESSMENT INFORMATION, FINAL RULE FEREAC 47,26992,82 EPA TSCA Section 8(d) unpublished health/safety studies On EPA IRIS database EPA TSCA TEST SUBMISSION (TSCATS) DATA BASE, SEPTEMBER 1997 NIOSH CURRENT INTELLIGENCE BULLETIN 46, 1986 NIOSH Analytical Method, 1994: Methylene chloride, 1005 NIOSH Analytical Method, 1996: Volatile organic compound, 2549 NTP Carcinogenesis Studies (inhalation);clear evidence:mouse,rat NTPTR* NTP-TR-306,86 NTP 7th Annual Report on Carcinogens, 1992 : anticipated to be carcinogen OSHA ANALYTICAL METHOD #ID-59 IRRITATION EFFECTS JOURNAL REFERENCES: EJTXAZ European Journal of Toxicology and Environmental Hygiene. Paris, France V.7-9, 1974-76. For publisher information, see TOERD9. TXCYAC Toxicology. Elsevier Scientific Pub. Ireland, Ltd., POB 85, Limerick, Ireland V.1- 1973- 85JCAE Prehled Prumyslove Toxikologie; Organicke Latky, Marhold, J., Prague, Czechoslovakia, Avicenum, 1986 MUTATION EFFECTS JOURNAL REFERENCES: CRNGDP Carcinogenesis (London). Oxford Univ. Press, Pinkhill House, Southfield Road, Eynsham, Oxford OX8 1JJ, UK V.1- 1980- EMMUEG Environmental and Molecular Mutagenesis. Alan R. Liss, Inc., 41 E. 11th St., New York, NY 10003 V.10- 1987- EVSRBT Environmental Science Research. Plenum Pub. Corp., 233 Spring St., New York, NY 10013 V.1- 1972- ITCSAF In Vitro. Rockville, MD V.1-20, 1965-85. For publisher information, see ICDBEO. MUREAV Mutation Research. Elsevier Science Pub. B.V., POB 211, 1000 AE Amsterdam, Netherlands V.1- 1964- TCMUD8 Teratogenesis, Carcinogenesis, and Mutagenesis. Alan R. Liss, Inc., 41 E. 11th St., New York, NY 10003 V.1- 1980- TECSDY Toxicological and Environmental Chemistry. (Gordon & Breach Science Pub. Inc., 1 Park Ave., New York, NY 10016) V.3 3/4- 1981- REPRODUCTIVE EFFECTS JOURNAL REFERENCES: TXAPA9 Toxicology and Applied Pharmacology. Academic Press, Inc., 1 E. First St., Duluth, MN 55802 V.1- 1959- TUMORIGENIC EFFECTS JOURNAL REFERENCES: FAATDF Fundamental and Applied Toxicology. Academic Press, Inc., 1 E. First St., Duluth, MN 55802 V.1- 1981- NTPTR* National Toxicology Program Technical Report Series. Research Triangle Park, NC 27709 No.206- TOXICITY EFFECTS JOURNAL REFERENCES: ABHYAE Abstracts on Hygiene. Bureau of Hygiene and Tropical Diseases, Keppel St., London WC1E 7HT, UK V.1- 1926- AGGHAR Archiv fuer Gewerbepathologie und Gewerbehygiene. Berlin, Ger. V.1-18, 1930-61. For publisher information, see IAEHDW. AHBAAM Archiv fuer Hygiene und Bakteriologie. Munich, Fed. Rep. Ger. V.101-154, 1929-71. For publisher information, see ZHPMAT. ENVRAL Environmental Research. Academic Press, Inc., 1 E. First St., Duluth, MN 55802 V.1- 1967- ESKGA2 Eisei Kagaku. Hygienic Chemistry. Nippon Yakugakkai, 2-12-15 Shibuya, Shibuya-ku, Tokyo 150, Japan V.1- 1953- FAONAU FAO Nutrition Meetings Report Series. Rome, Italy No.?-57, 1948-77. Discontinued. FLCRAP Fluorine Chemistry Reviews. Marcel Dekker, 270 Madison Ave., New York, NY 10016 V.1- 1967- GISAAA Gigiena i Sanitariya. For English translation, see HYSAAV. V/O Mezhdunarodnaya Kniga, 113095 Moscow, USSR V.1- 1936- HBTXAC Handbook of Toxicology, 4 vols., Philadelphia, W.B. Saunders Co., 1956-59 JIHTAB Journal of Industrial Hygiene and Toxicology. Cambridge, MA V.18-31, 1936-49. For publisher information, see AEHLAU. NIHBAZ National Institutes of Health, Bulletin. Bethesda, MD NTIS** National Technical Information Service. Springfield, VA 22161 Formerly U.S. Clearinghouse for Scientific & Technical Information. QJPPAL Quarterly Journal of Pharmacy & Pharmacology. London, UK V.2-21, 1929-48. For publisher information, see JPPMAB. SCIEAS Science. American Assoc. for the Advancement of Science, 1333 H St., NW, Washington, DC 20005 V.1- 1895- TPKVAL Toksikologiya Novykh Promyshlennykh Khimicheskikh Veshchestv. Toxicology of New Industrial Chemical Substances. For English translation, see TNICS*. Izdatel'stvo Meditsina, Moscow, USSR No.1- 1961- TXAPA9 Toxicology and Applied Pharmacology. Academic Press, Inc., 1 E. First St., Duluth, MN 55802 V.1- 1959- 34ZIAG Toxicology of Drugs and Chemicals, Deichmann, W.B., New York, Academic Press, Inc., 1969 OTHER MULTIPLE DOSE EFFECTS JOURNAL REFERENCES: AIHAAP American Industrial Hygiene Association Journal. AIHA, 475 Wolf Ledges Pkwy., Akron, OH 44311 V.19- 1958- APTOA6 Acta Pharmacologica et Toxicologica. Copenhagen, Denmark V.1-59, 1945-86. For publisher information, see PHTOEH DCTODJ Drug and Chemical Toxicology. Marcel Dekker, 270 Madison Ave., New York, NY 10016 V.1- 1977/78- FCTOD7 Food and Chemical Toxicology. Pergamon Press Inc., Maxwell House, Fairview Park, Elmsford, NY 10523 V.20- 1982- GISAAA Gigiena i Sanitariya. For English translation, see HYSAAV. V/O Mezhdunarodnaya Kniga, 113095 Moscow, USSR V.1- 1936- GTPZAB Gigiena Truda i Professional'nye Zabolevaniya. Labor Hygiene and Occupational Diseases. V/O Mezhdunarodnaya Kniga, 113095 Moscow, USSR V.1-36, 1957-1992. For publisher information, see MTPEEI NTIS** National Technical Information Service. Springfield, VA 22161 Formerly U.S. Clearinghouse for Scientific & Technical Information. NTPTR* National Toxicology Program Technical Report Series. Research Triangle Park, NC 27709 No.206- TOLED5 Toxicology Letters. Elsevier Science Pub. B.V., POB 211, 1000 AE Amsterdam, Netherlands V.1- 1977- TXAPA9 Toxicology and Applied Pharmacology. Academic Press, Inc., 1 E. First St., Duluth, MN 55802 V.1- 1959- REVIEWS JOURNAL REFERENCES: DTLVS* The Threshold Limit Values (TLVs) and Biological Exposure Indices (BEIs) booklet issues by American Conference of Governmental Industrial Hygienists ACGIH, Cincinnati, OH, 1996 FAZMAE Fortschritte der Arzneimittelforschung. Progress in Drug Research. Birkhauser Boston, Inc., c/o Springer-Verlag New York, 44 Hartz Way, Secaucus, NJ 07094 V.1- 1959- IMEMDT IARC Monographs on the Evaluation of Carcinogenic Risk of Chemicals to Man. WHO Publications Centre USA, 49 Sheridan Ave., Albany, NY 12210 V.1- 1972- IMSUDL IARC Monographs, Supplement. WHO Publications Centre USA, 49 Sheridan Ave., Albany, NY 12210 No.1- 1979- STANDARDS & REGULATIONS JOURNAL REFERENCES: CFRGBR Code of Federal Regulations. U.S. Government Printing Office, Supt. of Documents, Washington, DC 20402 DTLVS* The Threshold Limit Values (TLVs) and Biological Exposure Indices (BEIs) booklet issues by American Conference of Governmental Industrial Hygienists ACGIH, Cincinnati, OH, 1996 FEREAC Federal Register. U.S. Government Printing Office, Supt. of Documents, Washington, DC 20402 V.1- 1936- NTP, NIOSH, EPA STATUS JOURNAL REFERENCES: FEREAC Federal Register. U.S. Government Printing Office, Supt. of Documents, Washington, DC 20402 V.1- 1936- NTPTR* National Toxicology Program Technical Report Series. Research Triangle Park, NC 27709 No.206- DATA PRESENT: Irritation Effects; Mutation Effects; Reproductive Effects; Tumorigenic Effects; Toxicity Effects; Human Toxicity Effects; Other Multiple Dose Effects; Reviews; Standards and Regulations; NIOSH Criteria Documents; NTP, NIOSH, EPA Status |
